Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Whole tissue: Total RNA was isolated from the atrial tissue using Trizol according to the manufacturer's protocol (Sigma Aldrich), DNaseI treated and further purified using Nucleospin RNeasy MinElute Kit (Qiagen). PCM-1 samples: nuclei were extracted from snapfrozen samples, and stained with PCM-1 rabbit polyclonal antibody (Sigma) for FACS of specifically cardiomyocyte nuclei. Adult tissue: NuGEN's Ovation RNA-seq V2 kit. Fetal: Truseq Stranded Total RNA Library Preparation Kit (Illumina, Part# 15031048 Rev. E)